Itaq universal sybr green supermix is a 2x concentrated ready to use reaction master mix optimized for dye based quantitative pcr qpcr on any real time pcr instrument. 1 generating consistent amplification across a wide dynamic range is fundamental to qpcr methodology and use of the δδct method for gene expression analysis. Quantitative rt pcr protocol sybr green i 5 3 running 3 agarose gel after qrt pcr 4 analyze qrt pcr data.
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